TABLE 4.

Effect of urine concentration by ultrafiltration on inhibition ELISA results over time with urine from rabbits infected with C. albicans strain 36B

DayMean % inhibition ± SE (n)a% Increase by 10× concentration
1 × urine10 × urine
00 ± 0 (3)0.1 ± 2.5 (3)
112.0 ± 2.3 (3)13.9 ± 3.2 (3)15.8
216.7 ± 2.6 (3)17.8 ± 2.7 (6)6.6
343.1 ± 2.2 (4)67.3 ± 6.5 (4)b56.1b
443.8 ± 2.3 (3)71.2 ± 1.0 (3)c62.6c
4 (CC)d49.8 ± 2.0 (3)87.3 ± 0.6 (3)c,e75.3c
  • a Mean percent ELISA inhibition ± SE for the number of experiments in parentheses; the cutoff value for a positive ELISA result with unconcentrated urine was 13.5% (see Materials and Methods).

  • b Significant increase in ELISA inhibition compared with 1× concentration results (P < 0.02).

  • c Significant increase in ELISA inhibition compared with 1× concentration results (P < 0.001).

  • d CC, clean-catch urine.

  • e No significant difference in ELISA inhibition was noted for unconcentrated clean-catch urine compared with unconcentrated non-clean-catch urine; there was a significant difference between ELISA inhibition by 10× clean catch urine and that by 10× non-clean-catch urine (P < 0.001).