TABLE 3.

Detection of EBO-R NP from clinical specimens

SampleMonkey no.Titer by antigen capture ELISAaResult by:
Res2-6C8Res2-1D83-3DbRT-PCRcIHCd
Serum26121604020++
2866320320160+ND
Liver287740<1010NDeND
28821604040++
Spleen287732016080NDND
28828020<10ND+
288516040<10+ND
  • a Antigen titer in the specimens. The antigen titer in the ELISA was determined as the reciprocal of the highest dilution showing a positive reaction. The titer was determined by a single test. The cutoff OD value was 0.2.

  • b Ebola virus species cross-reactive MAb (29).

  • c RT-PCR with primers RES-Nf2 and RES-Nr2.

  • d IHC, immunohistochemistry for the detection of Ebola virus NP (14).

  • e ND, not done.