TABLE 1.

Removal of OspC antibodies from early Lyme disease sera by adsorption with OspC or OspC-Dra

Serum sampleOspC ELISA reactivitya after adsorption with:
Undiluted serumPurification tagbOspCOspC-Dra
IgMIgGIgMIgGIgMIgGIgMIgG
120,48010,24020,48010,240160NDcNDND
281,9201,280163,8401,280NDND80ND
320,48032020,4802,560NDNDNDND
440,9605,12040,9605,120320ND80ND
55,1203202,560160NDNDNDND
620,48081,92020,48040,960ND8080ND
781,92020,48040,96010,240320NDNDND
  • a Reciprocal of last dilution with adsorbance value of ≥0.200 above that of the normal serum control. ELISA reactivity that remained after adsorption with OspC or OspC-Dra could be removed completely by additional passages over fresh columns.

  • b Serum passed through column containing purification tag only.

  • c ND, no ELISA reactivity detected.