TABLE 1.

Primers used for PCR of each of the seven members of the Vap family

VapPrimer 1Primer 2
VapA5′-GCCGGATCCACTAATGCGACCGTTCTT-3′5′-CATGAATTCCTAGGCGTTGTGCCA-3′
VapC5′-GCCGGATCCGCCAATGTAGTCGCTCCGTC-3′5′-CATGAATTCGCGAGCGTTTACCTTCCGAC-3′
VapD5′-GCCGGATCCACGGCGAGAAGACGAAAGTGT-3′5′-CATGAATTCTTGTTCCTCACGCAGCCCAC-3′
VapE5′-GCCGGATCCATAGCATTGGTTCTAATCGCA-3′5′-CATGAATTCTACTGCACTGCAGCCGGACT-3′
VapF5′-CACGGATCCAACAGATGTTGTGGCGATTGC-3′5′-CCTGAATTCTTCGTCCGCTTCTGCCAATC-3′
VapG5′-GCGAATTCCAGAAGAGGTACGTGGCATTG-3′5′-CTGGGATCCATGGTATCCACTACAGCAGC-3′
VapH5′-CATGGATCCGTGTACCAACGGCTGAAGCTC-3′5′-CCTGAATTCCACGTGATTCTTCGCTTGGAC-3′