TABLE 3.

Medians and interquartile ranges for cytokine measures in subjects with detectable cytokine responses

MarkerSubjects with detectable cytokine responses
HIV seronegativeHIV seropositive
AllVL <400VL 400-10,000VL >10,000
nMedianQ25-Q75nMedianQ25-Q75nMedianQ25-Q75nMedianQ25-Q75nMedianQ25-Q75
Unstimulated protein
    IL-2b5544.016-1039444.415-1392341.315-1184568.021-2212639.46-51
    IFN-γ5222.06-698117.06-541618.55-723725.09-1422813.56-22
    IL-42732.06-743922.06-751236.48-801411.05-831348.012-61
    IL-69555.019-14915369.020-2813432.517-1426387.014-3175685.227-268
    TNF-α5024.215-9611227.212-932627.214-535122.07-1023539.017-92
Stimulated protein
    IL-28754.030-19716549.021-1714042.123-1766752.020-1715850.720-193
    IFN-γ147178.039-739260266.060-66154358.077-64411520539-77191276.066-592
    IL-4448.54-38649.54-64159.06-652912.04-26206.04-69
    IL-6170301.669-598304333.574-65658276.183-799136344.056-638110352.093-621
    TNF-αc148367.6184-725276238.093-59950343.099-847126222.570-554100246.5100-618
Unstimulated mRNA
    IL-2204.02-4382.02-452.02-4202.02-4132.02-4
    IFN-γ343.02-8704.01-10176.02-20324.01-10214.02-8
    IL-4332.01-2441.81-2121.51-2192.01-4131.00.2-2
    TNF-α444.02-20874.02-20185.02-20413.22-40286.02-20
Stimulated mRNA
    IL-26110.04-409410.04-402220.04-204010.03-203220.04-60
    IFN-γ64200.040-1,000125200.040-60027200.060-1,00052200.033-40046250.0200-600
    IL-4392.01-4662.51-6173.02-6242.00.3-4254.02-8
    TNF-α5340.020-400100100.020-40026150.020-4004140.020-40033100.024-400
  • a The number (n) of subjects with detectable cytokine responses and median and interquartile range values for cytokine protein and mRNA levels are shown for HIV-seronegative and HIV-seropositive subjects. All HIV-seropositive subjects and HIV-seropositive subjects with viral loads (VL) of <400, 400 to 10,000, and >10,000 copies/ml were studied. Values for protein are in picograms per milliliter. Values for mRNA are in attomoles per 104 cells. Interquartile ranges are from Q25 (first quartile [25th percentile]) to Q75 (third quartile [75th percentile]).

  • b The values for the three HIV-positive groups with viral loads of <400, 400 to 10,000, and >10,000 copies/ml were significantly different (P = 0.04 by Kruskal-Wallis rank sum test).

  • c The values for the HIV-seronegative and HIV-seropositive (all) subjects (P = 0.003) and for all four groups (P = 0.01) were significantly different by the Kruskal-Wallis rank sum test.