TABLE 3.

Recovery of spiked cytokines in a multiplex assaya

CytokineCytokine recovery (pg/ml) at expected concn indicatedb
10 pg/ml100 pg/ml1,000 pg/ml
IL-1α10.5 ± 1.397 ± 61,017 ± 51
IL-1β10.1 ± 1.3102 ± 121,020 ± 47
IL-29.6 ± 1.1100 ± 81,003 ± 57
IL-417.0 ± 5.6116 ± 19998 ± 76
IL-512.1 ± 3.7108 ± 7991 ± 66
IL-610.4 ± 2.2102 ± 191,003 ± 58
IL-825.2 ± 16.197 ± 101,048 ± 87
IL-109.3 ± 2.2105 ± 51,014 ± 54
IL-12p7011.9 ± 3.1102 ± 11978 ± 60
IL-1311.7 ± 1.5105 ± 61,018 ± 31
IL-159.2 ± 1.194 ± 111,007 ± 47
IL-1726.7 ± 15.8108 ± 181,045 ± 100
IL-188.7 ± 1.294 ± 3.41,007 ± 81
TNF-α11.6 ± 2.3103 ± 121,042 ± 70
IFN-γ8.1 ± 3.291 ± 61,073 ± 120
  • a Known amounts of cytokines were spiked into RPMI culture medium. Samples were treated as unknown samples and determined in a multiplex assay. The mean ± standard deviation recoveries (picograms per milliliter) for the different amounts of each cytokine are shown.

  • b Six experiments were done for each spiked cytokine.