TABLE 1.

Reagents used for Bio-Plex immunoassaysa

CytokineRecombinant proteinCapture AbDetection Ab
Catalogue no.SourceCatalogue no.CloneSourceCatalogue no.CloneSource
IL-1αSD901BSAHC0912624B 3F2BSAHC0419840C 20B8BS
IL-1βM6058CLBM9313CLB/IL1B-8CLBM9313CLB/IL1B-4CLB
IL-2554603BD5550515344.111BD555040B33-2BD
IL-4204-IL-005R&DMAB6043010.211R&DBAF204PolyclonalR&D
IL-5PHC0055BSAHC0954JES1-39D10BSAHC0859JES1-5A10BS
IL-6M6077CLBM9316CLB/IL6-16CLBM9316PolyclonalCLB
IL-889/520NIBCSM9318CLB/IL8-1CLBM9318PolyclonalCLB
IL-10M6086CLBM9310B-N10CLBM9310B-T10CLB
IL-12p70219-IL-005R&DMAB61124945.11R&DBAF219PolyclonalR&D
IL-1394/622NIBCSM9313CLB/IL13-1CLBM9313CLB/IL13-1CLB
IL-15247-IL-005R&DMAB64734505.11R&DBAM24734593.11R&D
IL-17317-IL-050R&DMAB31741809.111R&DBAF317PolyclonalR&D
IL-18B001-5MBLD044-3125-2HMBLD045-6159-12BMBL
TNF-αM6145CLBM9323CLB/TNF-7CLBM9323CLB/TNF-5CLB
IFN-γ554616BD554548NIB42BD5545504S.B3BD
  • a The reagents listed were obtained from the sources indicated by the following abbreviations: BS, Biosource; CLB, Sanquin; NIBSC, National Institute for Biological Standards and Controls (Potters Bar, United Kingdom); BD, BD Biosciences; R&D, R&D Systems; MBL, Medical & Biological Laboratories (Naka-ku Nagoya, Japan). All capture antibodies are mouse or rat monoclonal reagents. Detection antibodies for IL-4, IL-6, IL-8, IL-12, and IL-17 are either sheep or goat; others are monoclonal mouse or rat. Ab, antibodies.