TABLE 1.

Primers and probe for the amplification of a 78-bp sequence within the bovine Mx1 gene (Genbank accession number AF047692 ) by TaqMan PCR

Primer or probeLength (nucleotide)Start nucleotideSequence (5′-3′) and fluorogenic labelsaMelting point (°C)
Forward primer191714AAC ACC TGA CCG CGT ACC A59
Reverse primer221791GCA CGA AGA ACT GGA TGA TCA A59
Probe241734FAM-CAG GAA GTC AGC ACC CGC ATC TCC-TAMRA69
  • a FAM, 6-carboxy-fluorescein; TAMRA, 6-carboxy-tetramethyl-rhodamine.