TABLE 1.

Primers used for real-time reverse transcription-PCR detection of GAPDH, IFN-γ, and IL-4 in BALF cells of adult horses

Target genePrimer nameSequence
GAPDHGAPDH F5′-GTAATCAACGGAAAGGCCATCA-3′
GAPDH R5′-CCTTTTGGCTCCACCCTTCAAG-3′
IFN-γIFN-γ F5′-AGGTCCAGCGCAAAGCAATAAG-3′
IFN-γ R5′-TTGCAGGCAGGATGACCACTAT-3′
IL-4IL-4 F5′-CCGAAGAACACAGATGGAAAGGA-3′
IL-4 R5′-TCACAGTACAGCAGGTCCCGTTT-3′