TABLE 1.

Sensitivity and specificity of ELISA for the detection of different immunoglobulin classes in urine and serum using L. donovani crude antigen or acetone-treated antigen

SampleAntigenIg classSensitivity for VLaSpecificitya,b
EHCNEHCMTBCLOD
UrineAcetone treatedIgG57/60 (95.0)59/59 (100)53/53 (100)54/59 (91.5)13/13 (100)20/23 (87.0)5/7 (71.4)
CrudeIgG58/62 (93.5)59/59 (100)53/53 (100)44/59 (74.6)12/13 (92.3)18/23 (78.3)5/7 (71.4)
IgM30/45 (66.7)12/13 (92.3)14/14 (100)4/7 (57.1)
IgA17/45 (37.8)13/13 (100)14/14 (100)3/7 (42.9)
SerumAcetone treatedIgG36/40 (90.0)54/54 (100)
CrudeIgG36/40 (90.0)54/54 (100)
  • a Number positive/total (percent).

  • b EHC, healthy controls from areas of endemicity; NEHC, healthy controls from areas of nonendemicity; M, malaria; TB, tuberculosis; OD, other diseases; −, not tested.