TABLE 1.

Evaluation of serum samples from healthy donors and patients with TB (smear positive and negative)a

Serum sample no.Patient statusReactivity by:
SmearELISA (OD450)
Individual antigenFusion
Tb Lysate38 kDaMtb11Mtb8Mtb48DPEPTbF10TbF6F10-DPEP-Mtb48TbF6 + DPEP
254004TB 2.139 0.965 0.102 0.173 0.296 1.591 0.482 1.01 1.666 2.859
258004TB++ 2.225 3.154 0.197 2.865 0.568 0.045 2.500 3.592 2.587 3.619
263004TB+++ 2.543 1.444 1.155 0.097 0.469 1.117 2.017 2.337 2.136 3.04
264004TB+ 2.147 0.615 0.242 0.059 0.708 0.233 0.318 1.028 0.699 1.716
266004TB++ 1.369 2.395 0.096 2.727 0.065 0.231 2.391 3.506 2.390 3.552
301004TB+++0.661 2.565 0.0490.02 0.397 0.135 1.781 3.184 1.718 3.227
322004TB 1.12 0.781 0.0460.024 0.692 0.152 0.632 1.409 1.109 2.234
546004TB+ 1.88 2.191 0.013 2.117 0.195 0.228 1.924 3.028 1.903 3.398
269004TB 1.518 2.217 0.0170.014 0.336 0.051 0.875 0.783 1.203 2.316
305004TB 1.755 1.201 0.0730.0680.1230.142 0.494 0.957 0.212 0.678
330004TB 2.047 1.87 0.0210.0560.1470.12 1.303 2.21 1.443 2.626
387004TB0.612 2.09 0.0890.0230.0230.04 1.228 2.051 1.231 2.395
261004TB 2.565 0.0730.0340.018 1.101 0.233 0.040 0.556 0.640 1.102
283004TB0.601 0.239 0.0730.015 0.604 0.344 0.048 1.059 0.791 1.658
298004TB+ 1.4 0.233 0.060.028 1.973 0.1410.063 2.463 1.138 2.802
300004TB+++ 1.048 0.0820.045−0.004 0.624 0.106 0.688 1.54 0.955 1.714
320004TB++0.4520.1140.033−0.025 0.757 0.179 0.056 1.034 0.744 1.291
445004TB++0.621 0.238 0.182 0.078 2.129 0.0860.127 1.657 1.433 1.918
274004TB 1.794 0.1010.012 3.054 0.035 0.354 2.376 3.483 2.599 3.573
312004TB 1.327 0.0760.011 0.763 0.2710.118 1.010 1.802 1.088 1.981
420004TB 2.133 0.1210.008 1.511 0.1210.041 1.906 3.214 2.051 3.322
276004TB+ 2.253 0.063 2.094 0.023 0.454 0.078 2.013 2.457 2.049 2.094
308004TB 1.433 0.072 2.859 0.0360.2420.142 2.509 3.067 2.522 2.898
416004TB 1.459 0.451 1.014 0.036 0.689 0.291 1.261 1.745 1.532 2.239
424004TB 0.992 0.113 1.561 0.0330.113 0.17 1.858 2.807 1.937 2.942
464004TB++ 0.876 0.234 0.753 0.021 0.318 0.098 0.914 1.524 1.127 1.887
495004TB++0.6850.054 1.236 0.030.0630.052 1.276 1.616 1.434 1.702
270004TB 1.269 0.0580.0220.0460.082 0.672 0.0210.1320.168 0.694
303004TB+++ 1.297 0.0570.021−0.004 0.81 0.604 0.011 0.923 1.167 1.508
326004TB 2.116 0.0770.042−0.019 0.386 0.634 0.122 0.975 0.572 1.564
410004TB+0.614 0.225 0.0630.030.198 1.135 0.133 0.392 1.671 2.483
411004TB 1.667 0.0960.0080.1110.042 0.433 0.103 0.241 0.566 1.053
421004TB 0.896 0.0680.029 0.222 0.062 0.78 0.176 0.307 0.989 1.522
528004TB0.16300.037−0.0510.074 1.359 0.0040.025 0.225 1.044
A6-1Donor0.3290.0420.0590.0240.1710.0250.0290.1470.1110.123
A6-2Donor0.1590.0550.0570.1010.1060.0320.0120.1040.0860.122
A6-3Donor0.6780.0330.0370.030.2080.0320.0130.0950.0980.11
A6-4Donor0.1080.040.0180.0310.0620.0240.0050.0560.0370.079
A6-5Donor0.2720.0660.0660.0910.1540.0510.0310.1630.0620.285
A6-6Donor0.0790.0310.0160.0150.0250.0610.0010.0780.0100.089
A6-7Donor0.1510.0190.0230.0020.0440.0560.0170.0990.0480.13
A6-8Donor0.3360.0560.0610.0390.0290.1490.0340.1160.0550.214
A6-9Donor0.1670.1580.0430.0380.0310.0260.1770.1530.1080.121
A6-10Donor0.0770.0220.0310.0160.0450.0410.0120.0510.0840.096
  • a A subset of TbF6-DPEP-positive sera was assayed with the recombinant antigens and lysate to demonstrate the restricted reactivity of some sera to discrete antigens. Values in italic and boldface type represent sera positive for a particular antigen. Boldface numbers outline examples of sera that are restricted in some cases to an individual antigen.