TABLE 2.

Monoclonal TCR transcriptsa

PatientSourceV familyV regionCDR3 regionJoining regionJ family
MZ-CC01-1CD8+ PBLVA 9SAMYYCAPASRSNTGKLIFGQGAJ 37
VA 19SAVYICAVDSRAAGNKLTFGGGAJ 17
VA 22SAVYFCALSPISSGSARQLTFGSGAJ 22
CD4+ TILVA 2SATYLCAVNTYSGNTPLVFGKGAJ 29
VA 5SATYLCALDQGDYKLSFGAGAJ 20
VA 6SAMYFCAMREGYSGNTPLVFGKGAJ 29
VA 7S2SASYLCAVREQAAGNKLTFGGGAJ 17
VA 12SAVYFCAPLTNAGKSTFGDGAJ 27
VA 13SGVYFCAVSGYSTLTFGKGAJ 11
VA 21SAVYFCAAKDQAGTALIFGKGAJ 15
VA 24SASYICVVPNTDKLIFGTGAJ 34
VB 5S8SALYLCASSFRIGYGYTFGSGBJ 1-2
VB 17TAFYLCASREWGDTEAFFGQGBJ 1.1
MZ-CC01-2CD8+ PBLVA 5SATYLCALDSRAAGNKLTFGGGAJ 17
VA 8S2SAVYFCAENTFNQGGKLIFGQGAJ 23
VA 17SATYFCAATYVNSGGGADGLTFGKGAJ 45
CD4+ TILVA 2SATYLCVVPDGINDMRFGAGAJ 43
VA 13SGVYFCAVERWARLMFGDGAJ 31
VA 20TAVYYCLVGPLMNSGGYQKVTFGIGAJ 13
VA 22SAVYFCALKGRNNNDMRFGAGAJ 43
VA 23SATYLCAVRSGAGSYQLTFGKGAJ 28
TumorVA 16SALYFCAVRDHSSGGYNKLIFGAGAJ 4
VB 7S1SALYLCASSQEGDLYEQYFGPGBJ 2-7
VB 15TALYFCATSDSDSGDTDTQYFGPGBJ 2-3
VB 23SALYFCASSQDNSYEQYFGPGBJ 2-7
MZ-CC01-3CD8+ PBLVA 13SGVYFCAVDAGYGNEKLTFGTGAJ 48
  • a cDNA was generated from CD4+ and CD8+ PBL, freshly harvested tumor tissue, and CD4+ TIL and tested for monoclonal TCR VA or VB transcripts as described in the text. TCR VA or VB families were characterized for monoclonality by direct sequencing of the amplified product from each TCR family. Note the large number of monoclonal TCR VA mRNA transcripts in CD4+ TIL from patient MZ-CC01-1. Monoclonal TCR transcripts detected in freshly harvested tumor tissue could not be detected in TIL or in PBL. Thus, individual TCR clonotypes could not be expanded in vitro. Short-term (7 days) in vitro culture of T cells (TIL) in the presence of autologous tumor and IL-7 resulted in an oligoclonal T-cell population.