TABLE 3.

Immunohistochemical staining for components at the local site in study subjects

MediatorStudy day% Mediator (range)a in:
AdultsChildren
MPO21.45 (1.08-8.87)*†‡1.21 (1.13-2.23)‡
70.01 (0.01-0.05)0.21 (0.05-0.35)‡
300.01 (0.005-0.01)0.06 (0.01-0.28)
HCb0.01 (0.002-0.02)0.01 (0.01-0.07)
Lf21.16 (0.53-1.71)*†‡1.18 (0.85-1.79)‡¶
70.02 (0.01-0.23)‡0.51 (0.22-1.63)‡
300.05 (0.01-0.12)0.30 (0.18-0.95)‡
HC0.003 (0.001-0.01)0.01 (0.01-0.42)
iNOS23.77 (3.13-5.61)†‡3.1 (2.3-5.3)†‡
300.97 (0.28-1.64)2.0 (1.31-3.26)‡
HC1.23 (1.11-1.76)0.16 (0.03-1.51)
  • a The average studied areas for each section were 9.3 × 105 μm2 for the duodenal biopsies and 10.2 × 105 μm2 for the rectal biopsies. Quantification of immunoreaction-positive areas relative to the total tissue section was determined by a computerized image-analyzing technique, and the results are expressed as the percentage of the ratio of positive area to the total area. Paraffin-embedded duodenal sections were studied in adults and in rectal sections in children in the study subjects. The Mann-Whitney U test was used to compare the statistical significance (P ≤ 0.05) in comparison between patients at the acute stage and at convalescence at day 7 (*) or day 30 (†) or between day 7 and day 30 (¶) after the onset of disease in patients or between patients at different stages of the disease and healthy controls (‡). Data are given as medians with ranges (25th and 75th percentiles) in parentheses.

  • b HC, healthy controls.