TABLE 1.

Efficiency of purified antibodies to block specific binding

CytokineMean % (range) cytokine-positive cells
None0.5 μg2 μg5 μg10 μg
IL-210037.2 (33-45)7.3 (4.5-9.4)1 (0.7-1.5)0.3 (0.1-0.5)
IFN-γ10099.8 (94-110)50.7 (29-71)6 (4.4-8.5)1.4 (0.1-2.4)
IL-610016.9 (12-26)5.3 (4.3-7.3)2.4 (1.9-3.3)1.8 (1-2.4)
IL-810066 (55-74)29.2 (23-35)1.1 (0.7-1.8)0.2 (0.1-0.3)
  • a Stimulated cells were preincubated with increasing concentrations of purified anticytokine antibodies (0.5, 2, 5, and 10 μg) before the addition of 0.5 μg of fluorochrome-labeled antibodies of an identical specificity. Values were obtained from three different experiments. The percentage of cytokine-positive cells is reported compared to stimulated cells which were incubated without purified anticytokine antibodies (set as 100%).