TABLE 1.

Scoring of intestinal lesionsa

Score (lesion severity)Lamina propria infiltration by lymphocytes and macrophagesEpithelial gland dilatationEpithelial cell hyperplasia
0 (normal)NormalNormalNormal
1 (minimal)Low numbers of lymphocytes and macrophages within lamina propria, <5 cells/field of viewOne or more glands dilated but empty, lined by normal epitheliumFocal area of glands lined by tightly packed enterocytes
2 (mild)5-10 cells/field of viewOne or more glands dilated, most empty or containing cellular debris, lined by normal epitheliumMultifocal areas of slightly elongated glands lined by tightly packed enterocytes
3 (moderate)10-20 cells/field of view, mild expansion of space between glands by cellular infiltrateMultifocal dilated glands, most containing cellular debris; multifocal glands lined by a flattened epitheliumMultifocal areas of elongate and slightly tortuous glands lined by tightly packed enterocytes with several mitotic figures
4 (marked)20-50 cells/field of view, glands widely separated by expanded lamina propria due to cellular infiltrateMultifocal dilated glands, most lined by flattened or denuded basement membrane and containing cellular debrisMultifocal, coalescent areas of elongate, tortuous glands, lined by tightly packed enterocytes with numerous mitotic figures
5 (severe)Inflammatory cells efface normal lamina propriaDiffuse gland dilatation with glands lined by flattened epithelium or denuded basement membrane and containing cellular debrisDiffuse elongate and irregular tortuous glands lined by tightly packed enterocytes with numerous mitotic figures
  • a Tissues examined and scored included the ileum, cecum, and colon of TCRα mice. For lamina propria infiltration, sections were scored at a final magnification of ×200.