TABLE 3.

Effects of Ebola virus infection and LPS stimulation on cytokine and chemokine RNA production in nonhuman primate alveolar macrophages

TimeTreatmentMean ± SEM % increase in template RNA fora:
IL-1βIL-6IL-8MIP-1αTNF-α
T0Mock infected11111
Infected1.9 ± 0.47b54.16 ± 34.93b1.87 ± 0.22b3.91 ± 0.59b1.19 ± 0.41
T6LPS52.35 ± 6.00b19,533.49 ± 6,432.60b24.81 ± 5.50b98.30 ± 17.24b60.10 ± 23.94b
Mock infected0.47 ± 0.050.71 ± 0.150.20 ± 0.033.52 ± 2.701.11 ± 0.86
Infected0.83 ± 0.3100.63 ± 0.110.41 ± 0.05b3.43 ± 1.370.28 ± 0.07
T12LPS41.82 ± 6.23b24,999.04 ± 8,976.29b25.71 ± 6.50b144.23 ± 58.92b162.31 ± 127.46b
Mock infected1.53 ± 1.20245.14 ± 229.050.35 ± 0.279.02 ± 7.375.51 ± 5.15
Infected0.44 ± 0.191.04 ± 0.390.51 ± 0.083.80 ± 1.143.32 ± 2.63
T24LPS18.57 ± 5.10b14,218.51 ± 9,022.08b12.69 ± 4.30b67.60 ± 28.6b58.87 ± 53.02b
Mock infected0.04 ± 0.022.53 ± 1.150.01 ± 0.000.60 ± 0.140.05 ± 0.05
Infected6.44 ± 4.19b5,864.48 ± 5,858.41b5.49 ± 2.96b25.38 ± 17.58b67.54 ± 63.64b
  • a Data are for treated cells relative to T0 mock-infected cells over time in four separate experiments with alveolar macrophages from four separate cynomolgus monkeys.

  • b Value was significantly different (P < 0.05) from value for mock-infected cells at the same time point.