TABLE 1.

Oligonucleotide primers for PCR amplificationa

GeneSense primerAntisense primer
groEL5′-CAACCCCGGGAGCGAAAAATATTAAATATA-3′5′-GCTTGACGTCTAGTAGTCCATTCCTCGGC-3′
5′-CAACGATGGCAGCGAAAAATATTAAATATA-3′5′-GCTTAAGAGACTAGTAGTCCATTCCTCGGC-3′
omp25′-GATCGGATCCTCCAAACTCATGAGACGAGT-3′5′-CCTGCATGCTTAATACACGTGGGTATTTTC-3′
5′-GATCCCTATGTCCAAACTCATGAGACGAGT-3′5′-CCTTAGAATTTAATACACGTGGGTATTTTC-3′
  • a For each gene, the nucleotides in boldface are restriction sites within the degenerate primers (upper sequences) used for directional cloning in the pQE30 vector: groEL sense, SmaI; groEL antisense, PstI; omp2 sense, BamHI; omp2 antisense, SphI. The underlined nucleotides represent translational start codons (sense primers) and stop codons (antisense primers) in the wild-type gene (lower sequences).