Table 1.

Summary of CCR5 Δ32 genotype results per specimen type

Specimen type (n) and assayNo. of genotype results/total tests per genotypea% of genotypes identified
WT/WTWT/Δ32Δ32/Δ32
PBMC (21)
 Standard NASBA16/164/41/1100
 Basic Kit16/164/41/1100
 DNA PCR16/164/41/1100
Whole blood (21)
 Standard NASBA13/137/71/1100
 Basic Kit13/137/71/1100
 DNA PCR13/137/71/1100
Dried blood spots (21)
 Standard NASBA13/137/71/1100
 Basic Kit13/137/71/1100
 DNA PCRb 9/137/71/180.95
Buccal scrapings (21)
 Standard NASBA17/173/31/1100
 Basic Kit17/173/31/1100
 DNA PCRc 8/170/31/142.8
Plasma (63)
 Basic Kit56/566/61/1100%
 DNA PCRd 0/150/40/10.00
  • a CCR5 genotypes: WT/WT, homozygous WT alleles; WT/Mut, heterozygous WT allele and Mut allele; Mut/Mut, homozygous Mut alleles.

  • b First-round DNA PCR gave positive results for 12 of 21 samples (including 3 that were weakly positive); second-round PCR using the template from the first-round PCR on the remaining 9 samples gave 5 more positive results, increasing the total number of positive results and percentage of genotypes identified to 17 of 21 and 80.95% respectively.

  • c First-round DNA PCR gave positive results for 8 of 21 samples (all weakly positive); second-round PCR on the 13 negative samples yielded one more positive result, increasing the total number of positive results and percentage of genotypes identified to 9 of 21 and 42.8%, respectively.

  • d DNA PCR results were negative for all samples after second-round PCR.