Table 1.

IFN-γ ELISPOT response following CD4 and CD8 T-cell depletion

Antigen and donorAdjusted SFC/106 PBMCsa
UnseparatedCD4 depletedCD8 depleted
VZV
 14812271277
 25151911207
Tetanus toxoid
 148135466
 25151120128
  • a Adjusted SFC counts represent the mean VZV or tetanus toxoid counts minus the mean counts for the appropriate control (MRC-5 antigen for VZV and medium for tetanus). All spot counts were determined from the means for replicate assay wells.