Table 4.

Comparison of adjusted VZV spot counts performed by human readers versus the ImmunoSpot Image Analyzera

Donor and bleed no.Human readersImmunoSpot computer
Mean SFC% RSDMean SFC% RSD
2617
 16325.8518.8
 25725.7436.4
 313414.111615.1
 415017.912412.7
3446
 13526.62912.4
 23824.23325.2
 33031.22717.7
 43532.22717.0
3802
 119529.71845.5
 227219.92526.9
 325923.723413.3
 432830.428716.5
 524127.72054.2
4574
 142118.74343.8
 232344.83196.2
 331318.33196.8
 434020.33514.4
 544423.851311.1
  • a Results are for frozen PBMCs collected at four or five different time points for each donor. Mean values and percent RSDs (coefficient of variation [standard deviation/mean] × 100) are based on counts obtained from five human readings and five ImmunoSpot computer readings for each donor bleed. SFC are per 106 PBMCs and represent the mean SFC for VZV antigen minus the mean SFC for MRC-5 control antigen.