Table 1.

Oligonucleotide primers and LightCycler hybridization probes

Target geneGenBank accession no.PrimerNucleotide positionsSequenceaSource
gyr D10489 gyrU219–239TTATGGTGCTGGGCAAATACA2
gyrL536–556CACCATGTAAACCACCAGATA2
gyrFL1484–512ATTTTAACTGTTTTACATGCTGGTGGTAA-FThis study
gyrLC1514–532Red640-TTTGGCGGTGGCGGATACA-phThis study
hla X01645 hlaU498–518AGAAAATGGCATGCACAAAAA2
hlaL874–895TATCAGTTGGGCTCTCTAAAA2
hlaFL1794–813CAGGAAAAATTGGCGGCCTT-FThis study
hlaLC1815–836Red640-TTGGTGCAAATGTTTCGATTGG-phThis study
  • a F, fluorescein; Red640, LightCycler-Red 640-N-hydroxysuccinimide ester; ph, 3′-phosphate.