Table 1.

Primers selected for SBV RT-PCR and seminested PCR

PrimeraSequence (5′ to 3′)Nucleotide positionsbAmplified product length (bp)
SB1 fACC AAC CGA TTC CTC AGT AG221–240487
SB2 rCCT TGG AAC TCT GCT GTG TA689–708
SB3 r, s-nTCT TCG TCC ACT CTC ATC AC478–497276
SB6 fGTG GCA GTG TCA GAT AAT CC2351–2370834
SB7 rGTC AGA GAA TGC GTA GTT CC3166–3185
SB8 f, s-nGCG TAG ACC AGT GTT GTT GT2554–2573631
SB9 fACC GTT GTC TGG AGG TAG TT3856–3875267
SB10 rGCC GCA TTA GCT TCT GTA GT4104–4123
SB11 fATA TAC GGT GCG AGA ACT GC5707–5726897
SB12 rCTC GGT AAT AAC GCC ACT GT6585–6604
SB13 f, s-nCTG GAT GAG AGC GAA TGA AG6157–6176447
SB14 fAAT GGT GCG GTG GAC TAT GG8038–8057597
SB15 rTGA TAC AGA GCG GCT CGA CA8616–8635
  • a f, forward; r, reverse; s-n, seminested.

  • b Nucleotide positions refer to the published SBV sequence 11 (GenBank accession no. AF092924).