Table 2.

Cytokine message analysisa

Harvest time and treatmentMean cytokine concn ± SD (amol/104 cells)
IL-2IL-4IFN-γTNF-α
4 h
 No stimulation0.00012 ± 0.00010.0004 ± 0.0000.00008 ± 0.000080.00002 ± 0.000
 PHA0.001 ± 0.00080.0002 ± 0.0000.003 ± 0.0040.0008 ± 0.001
 PMA0.002 ± 0.00080.0005 ± 0.00080.002 ± 0.0040.0009 ± 0.002
 PHA + PMA0.003 ± 0.0050.0004 ± 0.00080.004 ± 0.0060.007 ± 0.0008
24 h
 No unstimulation0.0002 ± 0.00010.0002 ± 0.0000.00008 ± 0.000070.00005 ± 0.00007
 PHA0.0004 ± 0.00060.003 ± 0.00030.002 ± 0.0030.0001 ± 0.00007
 PMA0.001 ± 0.0010.0002 ± 0.00030.003 ± 0.0040.0008 ± 0.0009
 PHA + PMA0.002 ± 0.0050.0003 ± 0.00040.0008 ± 0.0010.002 ± 0.003
  • a A total of 106 cells were harvested at the indicated time points. Data from 18 donors are shown.