Table 3.

Net discrimination power of rep-PCR fingerprints from 15 ECOR strains in relation to cycling regimen and use of single versus multiple cyclers

Fingerprints from cyclers A and B analyzed separately or combinedCycling regimenNet discrimination power (%)a
ERIC2 fingerprintsBOXA1R fingerprintsComposite fingerprints
SeparateStandardb,d 7.45.713.0
65-TDb,e 26.711.919.7
CombinedStandardc,d −5.2−6.1−1.1
65-TDc,e 25.1−0.219.6
  • a Larger values for net discriminating power (similarity index − differentiation index) indicate better differentiation. Six replicate fingerprints per strain were included in each analysis.

  • b With A separate from B, for standard versus 65-TD cycling, P < 0.001 (ERIC2), P > 0.10 (BOXA1R), and P = 0.03 (composite).

  • c With A and B combined, for standard versus 65-TD cycling, P < 0.001 (ERIC2 and composite) and P = 0.08 (BOXA1R).

  • d With standard cycling, for A separate from B versus A and B combined, P = 0.04 (ERIC2) and P < 0.001 (BOXA1R and composite).

  • e With 65-TD cycling, for A separate from B versus A and B combined, P > 0.10 (ERIC2 and composite) and P = 0.002 (BOXA1R).