Table 3.

Cytokine recovery from sponges using final protocol

ProteinMolecular mass (kDa)a% Recoveryb
IL-1β17.592.0 ± 5.0
IL-21585.3 ± 6.9
IL-415–195.0 ± 0.7
IL-51890.3 ± 6.8
IL-620.850.5 ± 2.1
IL-8858.7 ± 3.7
IL-1018.755.1 ± 4.3
IL-127585.9 ± 3.1
IFN-γ34–500
GM-CSF18–3263.3 ± 2.0
IgG150104.0 ± 6.3
IgA16098.3 ± 5.6
  • a From reference 2.

  • b Determined by comparing ELISA quantitation of extracted cytokine to stock solution of cytokine not absorbed on sponges. Data are means ± standard deviations for three sponges.