Table 2.

Cytokine recovery from sponges after a 30-min soak at room temperature or 4°Ca

Protein% Recovery at:
Room temp4°C
IL-260.1 ± 5.255.1 ± 4.3
IL-400
IL-593.7 ± 12.3488.1 ± 10.9
IL-634.8 ± 12.640.5 ± 4.4
IL-1029.6 ± 12.654.2 ± 7.4
IL-1283.5 ± 11.478.8 ± 5.4
GM-CSF62.7 ± 10.660.0 ± 3.9
IFN-γ00
IgA99.5 ± 10.398.3 ± 5.6
  • a Data are means ± standard deviations from three experiments using one wash with high-salt extraction buffer followed by centrifugation at 16,000 × g.