Table 1.

Best conditions for propagating HEV strain G93-2 in A549 cells

Test groupCulture conditionsCPEaTCID50/0.025 ml
Mg2+ concn (mmol/liter)pHInoculum dose (MOI)
106.80.25++4.2
207.20.025+3.5
307.60.00251.5
4306.80.025++++5.67
5307.20.0025++++5.83
6307.60.25++++5.23
7606.80.00252.0
8607.20.25+++5.0
9607.60.0252.5
  • a −, no cells showing CPE; +, 25% of the cells show CPE; ++, 50% of the cells show CPE; +++, 75% of the cells show CPE; ++++, 100% of the cells show CPE.