Table 1.

Results for the performance of the two H. hepaticus-specific ELISAs

ELISAaELISA resultNo. (%) of mice with the indicatedHelicobacter infection status
Helicobacterfree (n = 41)H. hepaticusb(n = 44)H. hepaticusc(n = 7)H. bilisc (n = 7)H. muridarumc (n = 7)
RecombinantPositive1 (2)29 (66)7 (100)0 (0)0 (0)
Negative40 (98)15 (34)0 (0)7 (100)7 (100)
Detergent extractPositive1 (2)39 (89)7 (100)0 (0)0 (0)
Negative40 (98)5 (11)0 (0)7 (100)7 (100)
  • a The recombinant-antigen-based ELISA used the fusion protein, GST-MAP18, as the antigen, and the detergent extract-based ELISA used a detergent extract of H. hepaticusas the antigen. Sera from mice free of Helicobacterinfection, naturally infected with H. hepaticus, or experimentally inoculated with H. hepaticus, H. bilis, or H. muridarum were tested.

  • b Mice naturally infected with H. hepaticus.

  • c Mice experimentally inoculated with H. hepaticus, H. bilis, or H. muridarum.