Table 2.

Inhibitory effects of CI-959 and CI-959-A on proliferation of transformed T cells

CompoundIC50(μM)a
HTLV-I-infected cell lineT-cell lymphoma line
HT-1HT-2HT-3JurkatCCRF-CEMMOLT-4
CI-959>10.0>10.0>10.0>10.0>10.0>10.0
CI-959-A>10.0>10.0>10.09.9 ± 0.38.2 ± 0.5>10.0
  • a The 50% inhibitory concentrations (IC50s) of CI-959 and CI-959-A were determined by quantification of the inhibition of proliferation of HTLV-I-infected CD4+ cell lines and T-cell lymphoma lines. The proliferation of cell lines cultured for 4 days in the absence of compounds was 6,728 ± 512 cpm for HT-1 (2 × 104/well), 1,304 ± 1,859 cpm for HT-2 (2 × 104/well), 7,135 ± 990 cpm for HT-3 (2 × 104/well), 14,541 ± 7,618 cpm for Jurkat (2 × 104/well), 10,990 ± 4,619 cpm for CCRF-CEM (2 × 104/well), and 19,185 ± 8,003 cpm for MOLT-4 (2 × 104/well). Values are means ± standard deviations for three separate experiments.