Table 1.

Inhibitory effects of CI-959 and CI-959-A on primary proliferative response of T cells

CompoundEC50(μM)a
PHAAllogeneic PBMCsAllogeneic cultured DCsAutologous EBV-infected B cellsAutologous HTLV-I-infected T cells
CI-959>4.0>4.0>4.0>4.0>4.0
CI-959-A0.51 ± 0.240.52 ± 0.040.65 ± 0.100.52 ± 0.010.62 ± 0.05
  • a The EC50s of CI-959 and CI-959-A were determined by quantification of the inhibition of the proliferative response of T cells to various stimulators. The proliferative response of unseparated PBMCs (5 × 104/well) in the absence of compounds was 156,274 ± 112,391 cpm for PHA (2 μg/ml), 11,937 ± 3,514 cpm for allogeneic PBMCs (5 × 104/well), 14,771 ± 10,026 cpm for allogeneic cultured DCs (5 × 103/well), 28,165 ± 11,205 cpm for autologous EBV-infected B cells (3 × 104/well), and 30,306 ± 19,772 cpm for autologous HTLV-I-infected T cells (5 × 104/well). Values are means ± standard deviations for three to six independent donors.