Table 3.

Inhibitory effects of CI-959 and CI-959-A on differentiation of DCs from PBMCsa

CompoundConcn (μM)% Differentiation of DCs
CD83 DCsCD83+ DCs
None07.30 ± 3.502.70 ± 1.11
CI-95916.41 ± 1.302.50 ± 0.40
CI-959-A0.53.90 ± 1.31bNDc
1.02.25 ± 0.61d, e1.20 ± 0.65b, e
  • a The effect of CI-959-A on differentiation of DCs from PBMCs was determined. CD83DCs were differentiated by culturing PBMCs in the presence of GM-CSF and IL-4 for 7 days, and CD83+ DCs were differentiated by using GM-CSF, IL-4, and TNF-α. In each experiment, five independent cultures were used. The DC differentiation rate was calculated as follows: 100 × (number of DCs differentiated/number of PBMCs used). Means ± standard deviations are shown.

  • b P < 0.05 compared with no compound.

  • c ND, not determined.

  • d P < 0.01 compared with no compound.

  • e P < 0.05 compared with CI-959.