Table 4.

Dilution test and recovery calculations for the 1A7-IL-2 ELISAa

SampleDilution factorObserved FP concn (ng/ml)Expected FP concn (ng/ml)Recovery (%)
AUndiluted100.0
1/255.450.0110.8
1/431.925.0127.6
1/811.512.592.0
BUndiluted83.2
1/247.641.6114.4
1/424.120.8115.9
1/811.910.4114.4
CUndiluted73.3
1/241.936.7114.2
1/421.918.4119.0
1/811.29.2121.7
  • a Fusion protein (FP) levels spiked in sample buffer (A), 50% human serum (B), and 50% mouse serum (C) and its serial dilutions were assayed. Recovery was calculated as the ratio of values obtained by ELISA measurements to concentrations expected from the dilutions of primary samples. Every sample was assayed in duplicate.