Table 7.

Competition of ch14.18 and ch14.18-IL-2 fusion protein in the 1A7-IL-2 ELISAa

Total concn (ng/ml)Concn (ng/ml) of:Concn of ch14.18-IL-2 detected by ELISA (ng/ml)
ch14.18-IL-2ch14.18
100100098
100901092
100802081
100505047
100208022
10010909
1005955
10001000
1401004077
1801008046
42010032028
  • a ch14.18-IL-2 (lot 1) (100 ng/ml) was serially diluted in a buffer. ch14.18 was added to achieve a total concentration of 100 ng of protein per ml in each sample. Data are presented in nanograms of total fusion protein per milliliter in each sample. Then ch14.18-IL-2 (lot 1) (100 ng/ml) was mixed with increasing concentrations of ch14.18, so that the total amount of protein per well was increased. The mixture was run on the 1A7-IL-2 ELISA. Data are shown as concentrations of intact fusion protein and represent the means of two separate experiments.