Table 2.

Primer combinations and optimal temperatures for amplification of the genes encoding the MAP2 homologs ofE. canis and E. chaffeensis

Primer combinationDenaturingAnnealingExtension
Time (min)Temp (°C)Time (min)Temp (°C)Time (min)Temp (°C)
AB249-AB2511401721.594
AB282-AB2841401721.594
AB346-F171.5371.5721.594
AB347-AB1461.5371.5721.594
AB602-AB6081401721.594
AB620-AB6231471721.594
AB620-AB6241471721.594