Table 3.

1A7-IL-2 ELISA of ch14.18-IL-2 in serum relative to backgrounda

Buffer or serumMean OD405 for 1A7-IL-2 ELISA
Without proteinWith protein
PBS-Tween-milk0.0021.674
Mouse serum (100%)−0.0051.372
Human serum (%)
 1000.2420.997
  50−0.0121.495
  10−0.0091.673
   2−0.0061.732
   0.5−0.0021.759
   0.10.0011.587
  • a ch14.18-IL-2 fusion protein (lot 1) was added to PBS-Tween-milk, undiluted pooled mouse serum, or various dilutions of human serum and compared to buffer or serum alone as evaluated in the 1A7-IL-2 ELISA. Values listed indicate the relative OD405/492 readings obtained. Data presented are the means of duplicate samples.