Table 2.

Interassay variation of the 1A7-IL-2 ELISAa

Concn of ch14.18-IL-2 (ng/ml)ELISA result for ch14.18-IL-2
No. of samplesMean concn (ng/ml)CV (%)
115.09113.95.4
57.5960.27.3
28.8927.96.5
14.4914.63.5
7.297.34.2
3.693.69.9
0900
  • a The ch14.18-IL-2 fusion protein (lot 1) was serially diluted in a sample buffer at concentrations from 3.6 to 115.0 mg/ml, shown in the left column. The concentration of ch14.18-IL-2 was then quantitated by using the ELISA described in Fig.1A.