Table 1.

Monoclonal antibody combinationsa

Reagents (FITC/PE)CD no. (FITC/PE)Functional identity of relevant subsets
LeucoGATECD45/CD14Immunophenotype 3 cell differential (lymphocytes, monocytes, and neutrophils)
Isotype controlIgG1/IgG2Direct conjugate control for nonspecific staining
Leu-4/Leu-3CD3/CD4CD4+ T cells
Leu-4/Leu-2CD3/CD8CD8+ T cells
Leu-4/HLA-DRCD3/HLA-DRActivated (MHC class II expression) T-cells
Leu-4/Leu-11+ Leu-19CD3/CD16 + CD56NK cells (CD3 CD16+ CD56+)
Leu-4/Leu-12CD3/CD19T (CD3+ CD19) and B (CD3 CD19+) cells
  • a FITC, fluorescein isothiocyanate; PE, phycoerythrin; MHC, major histocompatibility complex; IgG1, immunoglobulin G1.