Table 1.

Analysis of serum samplesa

Sample no.Reciprocal ELISA titerSignal ratioSample concn (μg/ml)
Diluted (dilution)Original
9None (negative control serum)2.8600
7<322.000
8<321.9800
32561.32.3 (1:4)9
14,0960.3811.7 (1:4)47
24,0960.716.1 (1:9)55
44,0960.528.6 (1:4)34
516,3840.314.3 (1:29)415
616,3840.2218.1 (1:19)344
1116,3480.696.3 (1:29)183
1032,7680.518.7 (1:29)252
  • a The serum samples prepared at USAMRIID were tested at the Naval Research Laboratory. The samples were tested neat by competitive immunoassay and diluted and retested if necessary to obtain a signal ratio on a responsive portion of the standard curve (Fig. 3). These values are comparable to the ELISA titers determined at USAMRIID.