Table 5.

Evaluation of addition of various antigens from heterologous strains to IgG WB of whole-cell lysates of different strains

Basic test: WB with whole-cell lysate of straina:Additional antigen/strainLoss of specificity (%)Gain of sensitivity (%)b
PKa2p58/PKo1.46.3
p43/PKo6.48.5
p39/PKo1.45.3
p17/PKo3.56.3
p14/PKo0.73.2
p39/PBi0.03.2
OspC/PBi2.14.2
PKoOspC/PBi2.1c6.9c
OspC/PKa22.8c2.6c
PBip58/PKo2.86.9
p43/PKo5.77.4
p17/PKo3.57.9
p14/PKo0.74.8
  • a Results obtained in the preceding WB study (18); see Table 3.

  • b Only additional antigens leading to a gain of sensitivity of at least 3% are included.

  • c Results for criterion of a reactive OspC of strain PBi or at least two reactive bands among p83/100, p58, p43, p39, p30, OspC, p21, p17, and p14 of strain PKo for a positive WB.