TABLE 1.

Antigens of M. leprae identified by expression cloning using pooled LL/BL patient serad

AntigenNo. of clonesaSizes (kDa)TB homologue (% identity)DescriptionMean ELISA OD450 ± SDb
LL/BLNEC
ML0050/ML0049210, 9Rv3874 (40.0)Secreted; CFP-10, ESAT-60.52 ± 0.87c0.06 ± 0.01
Rv3875 (36.3)
ML0091423.7Rv3810 (52.7)Potentially secretedNDND
ML0317256.9Rv0440 (94.8)GroEL2, 60-kDa chaperonin 2NDND
ML0405240.7Rv3616c (62.7)Previously unknown2.31 ± 0.450.13 ± 0.02
ML0568518.3Rv1435c (38.9)Previously unknown0.34 ± 0.220.13 ± 0.04
ML1213380.1Rv1565c (76.5)Previously unknown0.51 ± 0.210.23 ± 0.04
ML2028334.8Rv1886c (88)Secreted; Ag85b2.29 ± 1.260.52 ± 0.21
ML2055629.5Rv1860 (66.8)Membrane protein1.87 ± 1.190.19 ± 0.05
ML2655236.3Rv0129 (82.0)Secreted; Ag85c2.82 ± 0.811.88 ± 0.28
ML0097135.4Rv3804c (82.0)Secreted; Ag85aNDND
ML1812150.5Rv1477 (78.8)Previously unknownNDND
ML2331226.5Rv3717 (82.0)Previously unknown2.09 ± 1.070.11 ± 0.06
ML2496324.2Rv0351 (68.6)DnaK, 70-kDa heat shock proteinNDND
  • a Number of different clones identified in the screen encoding the full or partial sequence of the same antigen.

  • b OD450 ± standard deviation in ELISA developed with protein A at a serum dilution of 1:200 (minimum of n = 6 for each measurement). ND, not done.

  • c ELISA results with ML0050-ML0049 fusion protein.

  • d n = 5.