Table 1.

Oligonucleotides used to generate recombinant fusion proteins of Omp2

OligonucleotidesapEV40-encoded fusion proteinSizeb (kDA)
Sense: 5′-GAATTCAGCGGGGGTATAGAGGC-3′ C. pneumoniae-Omp2aa23-aa93 10.0
Antisense: 5′-GGATCCTACTGAGCCTCTACAGG-3′
Sense: 5′-GAATTCAGCGGGAAGATAGAGG-3′ C. psittaci-Omp2aa23-aa94 10.9
Antisense: 5′-GGATCCTATGTAGCGTCTACTGG-3′
Sense: 5′-AGCGGGGTGTTAGAGACC-3′ C. trachomatis-Omp2aa23-aa84 9.8
Antisense: 5′-GGATCCTTATTTAGGTCCTGTAGCTTTAG-3′
Sense: 5′-CAGGATTCTTGCTTTGGC-3′ C. trachomatis-Omp2aa87-aa547 53.7
Antisense: 5′-CGGATCCTTAATAGATGTGTGTATTCTCTG-3′
Sense: 5′-AGCGGGGTGTTAGAGACC-3′ C. trachomatis-Omp2aa23-aa182 20.6
Antisense: 5′-CGGGATCCTTAAGGTTTTACCCATACAG-3′
Sense: 5′-CGCTTAGGACAAGGCG-3′ C. trachomatis-Omp2aa167-aa437 32.5
Antisense: 5′-CGGGATCCTTAATGAGTAGCAGCAACTCC-3′
Sense: 5′-TGCGCAGAAGCGACAA-3′ C. trachomatis-Omp2aa420-aa547 17.1
Antisense: 5′-CGGATCCTTAATAGATGTGTGTATTCTCTG-3′
  • a Underlined sequences are ofomp2 origin.

  • b Theoretical size of fusion protein.