Table 1.

Inhibition of antibody binding to pPGalMani i by modified pPGalMani i and by oligosaccharide fragmentsa

Inhibitorb50% Inhibitory concn (μM)Residues/ chain (n)
SaccharideGalactofuran chains
pPGalMani i 0.161.620
pPGalMani i in PBS0.131.320
PGalMan0.141.412
pPManNININ/A
PeptideNININ/A
pP(Gal2)Man9.8982
Galactofurano-oligo- saccharides
 Tetrasaccharide(s)55554
 Trisaccharide(s)1001003
 Disaccharide1801802
 1-O-β-CH3-d-Man3,600N/AN/A
Anionic saccharide1251252
  • a NI, no inhibition; N/A, not applicable.

  • b Molecular masses are as follows: pPGalManii, 65 kDa; PGalMan, 62 kDa; pPMan, 18.6 kDa; and pP(Gal)2Manii, 21.9 kDa.