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MICROBIAL IMMUNOLOGY

Cellular Immune Responses to Recombinant Antigens in Pregnant Women Chronically Infected with Toxoplasma gondii

A. F. Fatoohi, G. J. N. Cozon, T. Greenland, J. Ferrandiz, J. Bienvenu, S. Picot, F. Peyron
A. F. Fatoohi
1E. A. 3087 Parasitologie, Faculté de Médecine Lyon Nord
2Laboratoire de Parasitologie and Laboratoire d'Immunologie, Hôpital de la Croix-Rousse
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G. J. N. Cozon
1E. A. 3087 Parasitologie, Faculté de Médecine Lyon Nord
2Laboratoire de Parasitologie and Laboratoire d'Immunologie, Hôpital de la Croix-Rousse
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  • For correspondence: gregoire.cozon@chu-lyon.fr
T. Greenland
3Laboratoire d'Immunologie et de Biologie Pulmonaire, Hôpital Louis Pradel
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J. Ferrandiz
1E. A. 3087 Parasitologie, Faculté de Médecine Lyon Nord
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J. Bienvenu
4Laboratoire d'Immunologie Centre Hospitalier Lyon Sud, Lyon, France
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S. Picot
1E. A. 3087 Parasitologie, Faculté de Médecine Lyon Nord
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F. Peyron
1E. A. 3087 Parasitologie, Faculté de Médecine Lyon Nord
2Laboratoire de Parasitologie and Laboratoire d'Immunologie, Hôpital de la Croix-Rousse
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DOI: 10.1128/CDLI.9.3.704-707.2002
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    FIG. 1.

    Expression of CD25 on CD4+ T-cells from 22 chronically T. gondii-infected (filled diamonds) and 7 uninfected (open circles) pregnant women after a 7-day culture of whole blood with ST-Ag, recombinant antigens (GRA1, GRA6-CT, GRA7, and SAG1) and negative controls (PBS and GST).

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    FIG. 2.

    In vitro production of IFN-γ (filled diamonds) and IL-4 (open circles) after a 7-day culture of whole blood from 22 chronically T. gondii-infected pregnant women in the presence of soluble ST-Ag, recombinant antigen (GRA1, GRA6-CT, GRA7, and SAG1) or controls (PBS and GST). Cytokines were detected by using an ELISA (see Materials and Methods).

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Cellular Immune Responses to Recombinant Antigens in Pregnant Women Chronically Infected with Toxoplasma gondii
A. F. Fatoohi, G. J. N. Cozon, T. Greenland, J. Ferrandiz, J. Bienvenu, S. Picot, F. Peyron
Clinical and Diagnostic Laboratory Immunology May 2002, 9 (3) 704-707; DOI: 10.1128/CDLI.9.3.704-707.2002

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Cellular Immune Responses to Recombinant Antigens in Pregnant Women Chronically Infected with Toxoplasma gondii
A. F. Fatoohi, G. J. N. Cozon, T. Greenland, J. Ferrandiz, J. Bienvenu, S. Picot, F. Peyron
Clinical and Diagnostic Laboratory Immunology May 2002, 9 (3) 704-707; DOI: 10.1128/CDLI.9.3.704-707.2002
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