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MICROBIAL IMMUNOLOGY

A Latex Bead-Based Flow Cytometric Immunoassay Capable of Simultaneous Typing of Multiple Pneumococcal Serotypes (Multibead Assay)

M. K. Park, D. E. Briles, M. H. Nahm
M. K. Park
Departments of Pediatrics and of
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D. E. Briles
Department of Microbiology and Immunology, University of Alabama, Birmingham, Alabama 35294
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M. H. Nahm
Departments of Pediatrics and of
Pathology and Laboratory Medicine, University of Rochester, Rochester, New York 14642, and
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DOI: 10.1128/CDLI.7.3.486-489.2000
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    Fig. 1.

    Fluorescence analysis. (A) Red fluorescence and forward scatter of 15 different types of beads. (B) Histograms of green fluorescence for two types of beads. The shaded areas are histograms before inhibition; the open areas are histograms after inhibition. (C) The left panel shows that Hyp6BM9 can bind to 6A and 6B immobilized on the microtiter plate surface. The right panel shows inhibition of binding of Hyp6BM9 to 6B-coated latex particles in the presence of 6B PS or 6A PS in solution.

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  • Table 1.

    Serotyping results of 86 pneumococcal isolates with multibead assay

    SerotypeNo. of isolates
    AssignedaMultibead assay
    122
    344
    466
    522
    6A1010
    6B(7 − 1)6
    7F(3 − 1)2
    9N22
    9V33
    1488
    18C(5 − 1)4
    19A55
    19F(4 + 1)5
    22F33
    23F33
    Others(19 + 2)21
    • ↵a The numbers in parentheses reflect the adjustment in assignment that took place after the Quellung reaction, which was reperformed to address inconsistencies with the multibead assay results. For instance, seven isolates were originally labeled to be 6B. However, a repeat Quellung assay showed one isolate to have a different serotype. Thus, only six isolates were truly 6B.

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A Latex Bead-Based Flow Cytometric Immunoassay Capable of Simultaneous Typing of Multiple Pneumococcal Serotypes (Multibead Assay)
M. K. Park, D. E. Briles, M. H. Nahm
Clinical and Diagnostic Laboratory Immunology May 2000, 7 (3) 486-489; DOI: 10.1128/CDLI.7.3.486-489.2000

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A Latex Bead-Based Flow Cytometric Immunoassay Capable of Simultaneous Typing of Multiple Pneumococcal Serotypes (Multibead Assay)
M. K. Park, D. E. Briles, M. H. Nahm
Clinical and Diagnostic Laboratory Immunology May 2000, 7 (3) 486-489; DOI: 10.1128/CDLI.7.3.486-489.2000
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