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Performance of Competitive and Indirect Enzyme-Linked Immunosorbent Assays, Gel Immunoprecipitation with Native Hapten Polysaccharide, and Standard Serological Tests in Diagnosis of Sheep Brucellosis

C. M. Marín, E. Moreno, I. Moriyón, R. Díaz, J. M. Blasco
C. M. Marín
Unidad de Sanidad Animal, Servicio de Investigación Agraria, Diputación General de Aragón, Zaragoza, and
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E. Moreno
Programa de Investigación en Enfermedades Tropicales, Escuela de Medicina Veterinaria, Universidad Nacional, Heredia, Costa Rica
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I. Moriyón
Departamento de Microbiologı́a, Universidad de Navarra, Pamplona, Spain, and
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R. Díaz
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J. M. Blasco
Unidad de Sanidad Animal, Servicio de Investigación Agraria, Diputación General de Aragón, Zaragoza, and
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DOI: 10.1128/CDLI.6.2.269-272.1999
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ABSTRACT

Competitive and standard enzyme-linked immunosorbent assays (ELISAs), rose bengal (RB), complement fixation, and agar gel immunoprecipitation with native hapten (AGID-NH) were compared by using sera from Brucella-free, Brucella melitensis-infected, and B. melitensisRev1-vaccinated sheep. The most sensitive tests were indirect ELISA and RB, and the most specific tests were AGID-NH and competitive ELISA. We show that RB followed by AGID-NH is a simple and effective system for diagnosing sheep brucellosis.

  • Copyright © 1999 American Society for Microbiology
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Performance of Competitive and Indirect Enzyme-Linked Immunosorbent Assays, Gel Immunoprecipitation with Native Hapten Polysaccharide, and Standard Serological Tests in Diagnosis of Sheep Brucellosis
C. M. Marín, E. Moreno, I. Moriyón, R. Díaz, J. M. Blasco
Clinical and Diagnostic Laboratory Immunology Mar 1999, 6 (2) 269-272; DOI: 10.1128/CDLI.6.2.269-272.1999

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Performance of Competitive and Indirect Enzyme-Linked Immunosorbent Assays, Gel Immunoprecipitation with Native Hapten Polysaccharide, and Standard Serological Tests in Diagnosis of Sheep Brucellosis
C. M. Marín, E. Moreno, I. Moriyón, R. Díaz, J. M. Blasco
Clinical and Diagnostic Laboratory Immunology Mar 1999, 6 (2) 269-272; DOI: 10.1128/CDLI.6.2.269-272.1999
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