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Article

Detection of Antibodies to Human Immunodeficiency Virus Type 1 in Oral Fluids: A Large-Scale Evaluation of Immunoassay Performance

Timothy C. Granade, Susan K. Phillips, Bharat Parekh, Perry Gomez, Wendy Kitson-Piggott, Herbert Oleander, Bisram Mahabir, Waveney Charles, Stephanie Lee-Thomas
Timothy C. Granade
Division of AIDS, STD, and TB Laboratory Research, National Center for Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia 30333;
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Susan K. Phillips
Division of AIDS, STD, and TB Laboratory Research, National Center for Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia 30333;
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Bharat Parekh
Division of AIDS, STD, and TB Laboratory Research, National Center for Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia 30333;
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Perry Gomez
Ministry of Health and Environment, Nassau, Bahamas; and
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Wendy Kitson-Piggott
Caribbean Epidemiology Centre and
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Herbert Oleander
Ministry of Health and Environment, Nassau, Bahamas; and
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Bisram Mahabir
Ministry of Health, Port-of-Spain, Trinidad and Tobago
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Waveney Charles
Ministry of Health, Port-of-Spain, Trinidad and Tobago
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Stephanie Lee-Thomas
Division of AIDS, STD, and TB Laboratory Research, National Center for Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia 30333;
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DOI: 10.1128/CDLI.5.2.171-175.1998
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    Fig. 1.

    Correlation of S/CO ratios for OF specimens tested by GACELISA (A), OTC-M (B), and OTC-L (C) with S/CO ratios for specimens tested by serum EIA (Abbott). Data include all specimens initially HIV antibody reactive by any of the OF or serum EIAs and 250 HIV antibody-negative specimens.

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  • Table 1.

    Demographic data of study participants at the three survey sites

    CharacteristicNo. of subjects froma:
    Site 1Site 2Site 3All sites
    Sex
     Male1,5526348213,007
     Female4493676001,416
     Not reported112325
    Age (yr)
     <100145
     10–1981112155348
     20–297023945701,666
     30–397472904851,522
     40–49376132128636
     50–59844747178
     >6011243065
     Not reported122528
    Reason for providing specimen
     Blood donor2,000022,002
     STD14046241,029
     High-risk contact0243165408
     HIV contact0324072
     Intravenous drug use0151934
     Symptoms compatible with AIDS013723160
     Other0169546715
     Not reported122528
    Total subjects2,0021,0021,4444,448
    • ↵a Site 1, blood collection center, Port-of-Spain, Trinidad and Tobago; site 2, Queen’s Park Counseling Center, Port-of-Spain, Trinidad and Tobago; site 3, Comprehensive Health Clinic, Nassau, Bahamas.

  • Table 2.

    Sensitivities and specificities of serum and OF EIAs versus serum WBa

    Serum WB resultNo. of specimens
    GACELISAOTC-LOTC-M
    PNPNPN
    P474047044686
    N83,940333,91503,948
    • ↵a P, HIV-1 antibody positive; N, HIV-1 antibody negative.

  • Table 3.

    Comparison of detection of antibodies to HIV-1 proteins by WB in 474 matched serum and OF specimens

    WB antigenNo. (%) of HIV antibody-positive specimens
    SerumOF
    gp160474 (100)474 (100)
    gp120466 (98.3)470 (99.2)
    p66469 (98.7)462 (97.5)
    p55380 (80.1)365 (77.0)
    p51465 (98.1)451 (95.1)
    gp41471 (99.4)459 (96.8)
    p31457 (96.4)452 (95.4)
    p24469 (98.7)431 (90.9)
    p17435 (91.8)267 (56.3)
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Detection of Antibodies to Human Immunodeficiency Virus Type 1 in Oral Fluids: A Large-Scale Evaluation of Immunoassay Performance
Timothy C. Granade, Susan K. Phillips, Bharat Parekh, Perry Gomez, Wendy Kitson-Piggott, Herbert Oleander, Bisram Mahabir, Waveney Charles, Stephanie Lee-Thomas
Clinical and Diagnostic Laboratory Immunology Mar 1998, 5 (2) 171-175; DOI: 10.1128/CDLI.5.2.171-175.1998

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Detection of Antibodies to Human Immunodeficiency Virus Type 1 in Oral Fluids: A Large-Scale Evaluation of Immunoassay Performance
Timothy C. Granade, Susan K. Phillips, Bharat Parekh, Perry Gomez, Wendy Kitson-Piggott, Herbert Oleander, Bisram Mahabir, Waveney Charles, Stephanie Lee-Thomas
Clinical and Diagnostic Laboratory Immunology Mar 1998, 5 (2) 171-175; DOI: 10.1128/CDLI.5.2.171-175.1998
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