Detection of Antibodies to Human Immunodeficiency Virus Type 1 in Oral Fluids: A Large-Scale Evaluation of Immunoassay Performance

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Fig. 1.
Correlation of S/CO ratios for OF specimens tested by GACELISA (A), OTC-M (B), and OTC-L (C) with S/CO ratios for specimens tested by serum EIA (Abbott). Data include all specimens initially HIV antibody reactive by any of the OF or serum EIAs and 250 HIV antibody-negative specimens.

Table 1.

Demographic data of study participants at the three survey sites

Characteristic	No. of subjects from^a:
Characteristic	Site 1	Site 2	Site 3	All sites
Sex
Male	1,552	634	821	3,007
Female	449	367	600	1,416
Not reported	1	1	23	25
Age (yr)
<10	0	1	4	5
10–19	81	112	155	348
20–29	702	394	570	1,666
30–39	747	290	485	1,522
40–49	376	132	128	636
50–59	84	47	47	178
>60	11	24	30	65
Not reported	1	2	25	28
Reason for providing specimen
Blood donor	2,000	0	2	2,002
STD	1	404	624	1,029
High-risk contact	0	243	165	408
HIV contact	0	32	40	72
Intravenous drug use	0	15	19	34
Symptoms compatible with AIDS	0	137	23	160
Other	0	169	546	715
Not reported	1	2	25	28
Total subjects	2,002	1,002	1,444	4,448

↵a Site 1, blood collection center, Port-of-Spain, Trinidad and Tobago; site 2, Queen’s Park Counseling Center, Port-of-Spain, Trinidad and Tobago; site 3, Comprehensive Health Clinic, Nassau, Bahamas.

Table 3.

Comparison of detection of antibodies to HIV-1 proteins by WB in 474 matched serum and OF specimens

WB antigen	No. (%) of HIV antibody-positive specimens
WB antigen	Serum	OF
gp160	474 (100)	474 (100)
gp120	466 (98.3)	470 (99.2)
p66	469 (98.7)	462 (97.5)
p55	380 (80.1)	365 (77.0)
p51	465 (98.1)	451 (95.1)
gp41	471 (99.4)	459 (96.8)
p31	457 (96.4)	452 (95.4)
p24	469 (98.7)	431 (90.9)
p17	435 (91.8)	267 (56.3)