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Journal Article

Synthetic-peptide-based enzyme-linked immunosorbent assay for screening human serum or plasma for antibodies to human immunodeficiency virus type 1 and type 2.

L Gonzalez, R W Boyle, M Zhang, J Castillo, S Whittier, P Della-Latta, L M Clarke, J R George, X Fang, J G Wang, B Hosein, C Y Wang
L Gonzalez
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R W Boyle
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M Zhang
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J Castillo
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S Whittier
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P Della-Latta
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L M Clarke
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J R George
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X Fang
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J G Wang
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B Hosein
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C Y Wang
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ABSTRACT

A synthetic-peptide-based enzyme-linked immunosorbent assay (EIA) capable of screening for antibodies to both human immunodeficiency virus type 1 (HIV-1) and HIV-2 has been developed for use in blood banks and diagnostic laboratories. Microtiter wells are coated with two synthetic peptides, one corresponding to the highly conserved envelope region of HIV-1 and another corresponding to the conserved envelope region of HIV-2. Overall, sensitivity was 100% in 303 individuals diagnosed with AIDS and 96 individuals diagnosed with AIDS-related complex, 14.8% in a study of 500 high-risk group members, 99.9% in 600 EIA repeatedly reactive (RR)-HIV-1 Western blot (WB)-positive repository specimens, and 100% for 222 geographically diverse HIV-1 specimens and 216 confirmed HIV-2-positive specimens evaluated. The specificity was determined to be 99.72% for a total of 13,004 serum and plasma samples from random volunteer donors evaluated across five blood banks. Forty donors who were found to be EIA RR-WB indeterminate but nonreactive on the United Biomedical, Inc., test (UBI HIV 1/2 EIA) were prospectively followed as an additional measure of specificity. None of the 40 low-risk cases evolved into a positive WB pattern at follow-up. The sensitivity and specificity of this new assay are comparable to those of other Food and Drug Administration-licensed HIV-1 and HIV-1-HIV-2 assays that are currently available in the United States. The UBI HIV 1/2 EIA affords laboratories another choice in the detection of antibodies for HIV-1 and HIV-2 with a test based on an alternative antigen format.

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Synthetic-peptide-based enzyme-linked immunosorbent assay for screening human serum or plasma for antibodies to human immunodeficiency virus type 1 and type 2.
L Gonzalez, R W Boyle, M Zhang, J Castillo, S Whittier, P Della-Latta, L M Clarke, J R George, X Fang, J G Wang, B Hosein, C Y Wang
Clinical and Diagnostic Laboratory Immunology Sep 1997, 4 (5) 598-603; DOI:

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Synthetic-peptide-based enzyme-linked immunosorbent assay for screening human serum or plasma for antibodies to human immunodeficiency virus type 1 and type 2.
L Gonzalez, R W Boyle, M Zhang, J Castillo, S Whittier, P Della-Latta, L M Clarke, J R George, X Fang, J G Wang, B Hosein, C Y Wang
Clinical and Diagnostic Laboratory Immunology Sep 1997, 4 (5) 598-603; DOI:
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