Article Figures & Data
Figures
- FIG 1
Distribution of EPTB and results of the T-SPOT.TB test. (A) Distribution sites infected with M. tuberculosis in EPTB. (B) Spot-forming cells with the T-SPOT.TB test in the ATB and NATB groups. (C) Spot-forming cells with the two antigens in the ATB group and the PTB and EPTB subgroups.
- FIG 2
(A and B) Positive changes in pathology in ATB. (A) Pathology with a granuloma change. (B) Pathology with a caseous necrosis change. (C) Comparison of different tests for suspected ATB.
- FIG 3
T-SPOT.TB test performance results on pleural effusions. (A) Nil control. (B) Positive control. (C) Positive result for ESAT-6 antigen. (D) Positive result for CFP-10 antigen.
- FIG 4
ROC curve analysis of T-SPOT.TB in ATB diagnosis.
Tables
- TABLE 1
Clinical data for included patients
Parameter Value for (sub)group: ATB NATB PTB EPTB Total (n) 246 289 65 181 Age (yr) ± SD 45.81 ± 19.69 54.04 ± 17.43 45.15 ± 18.26 45.94 ± 21.62 Male [n (%)] 139 (56.5) 163 (56.4) 39 (60) 100 (55.25) Female [n (%)] 107 (43.5) 126 (43.6) 26 (40) 81 (44.75) Blood samples (n) 208 280 65 143 Body fluids (n) 38 9 0 47 CSF (n) 11 3 0 11 Pleural effusion (n) 17 4 0 17 Ascites (n) 10 2 0 10 T-SPOT.TB Median no. of SFCs 71 1 83 67.5 Positive/negative (n) 209/37 43/246 60/5 148/33 Sensitivity (%) (95% CI) 84.95 (80.5–89.0) NAa 92.31(83.77–97.13) 81.77(75.64–86.89) Specificity (%) (95% CI) NA 85.12 (79.9–89.2) NA NA ESAT-6 Median no. of SFCs 43 0 52 43 Positive/negative (n) 202/44 38/251 56/9 147/34 Sensitivity (%) (95% CI) 82.11 (76.95–86.5) NA 86.15 (76.11–93.03) 81.22 (75.03–86.41) Specificity (%) (95% CI) NA 86.85 (82.58–90.4) NA NA CFP-10 Median no. of SFCs 38 0 58 42 Positive/negative (n) 181/65 30/259 50/15 136/45 Sensitivity (%) (95% CI) 73.58 (67.8–78.8) NA 76.92 (65.55–85.95) 75.14 (68.45–81.02) Specificity (%) (95% CI) NA 89.62 (85.69–92.8) NA NA ↵a NA, not applicable.
- TABLE 2
Comparison of T-SPOT.TB to other test methods
Parameter Value T-SPOT.TB (n = 535) Pathologya (n = 176) Smear (n = 179) ESR (n = 437) ATB (n) Positive, 209; negative, 37 Yes, 78; no, 57 Detected, 12; undetected, 139 ≥20 mm/h, 141; <20 mm/h, 51 NATB (n) Positive, 43; negative, 246 Yes, 0; no, 41 Detected, 0; undetected, 28 ≥20 mm/h, 159; <20 mm/h, 86 Sensitivity (%) (95% CI) 84.95 (80.5–89.0) 57.78 (49.0–66.2) 7.95 (4.2–13.5) 73.44 (66.6–79.5) Specificity (%) (95% CI) 85.12 (79.9–89.2) 100 (98.5–100) 100 (87.7–100) 35.09 (29.1–41.4) PPV (%) (95% CI) 82.94 (77.7–87.4) 100 (95.4–100) 100 (73.5–100) 47 (41.2–52.8) NPV (%) (95% CI) 86.93 (82.4–90.6) 41.84 (95.4–100) 16.77 (73.5–100) 62.77 (54.1–70.9) PLR (95% CI) 5.71 (4.19–7.65) NAb NA 0.88 (0.78–1.00) NLR (95% CI) 0.18 (0.13–0.23) 0.42 (1.94–2.89) 0.92 (1.04–1.14) 1.32 (0.99–1.77) - TABLE 3
T-SPOT.TB test results from body fluid samples
Sample ATB (n) NATB (n) T-SPOT.TB (n) Sensitivity (%) Specificity (%) WBC count (median no. of cells) Positive Negative ATB NATB CSF 11 3 0 14 0 100 195 × 106/liter 68 × 106/liter Ascites 10 2 8 4 80 100 3,120 × 106/liter 3,590 × 106/liter Pleural effusion 17 4 15 6 82.35 75 2,980 × 106/liter 376 × 106/liter - TABLE 4
ROC curve results of T-SPOT.TB test for diagnosing ATB
Test Analysis results of ROC curves Diagnosis of active tuberculosis at different cutoffs AUC (95% CI) Cutoff (no. of SFCs) Sensitivity (%) Specificity (%) Youden indexa Cutoff (no. of SFCs) Sensitivity (%) Specificity (%) PPV T-SPOT.TB 0.906 (0.878–0.935) 14.5 80.9 91.3 0.72 32.5 70.3 97.9 97.1 ESAT-6 0.884 (0.852–0.916) 6.5 81.3 88.9 0.70 37.5 67.5 99.7 99.56 CFP-10 0.877 (0.845–0.908) 16.5 65.9 94.1 0.6 42.5 64.6 100 100 ↵a Youden index = 1 − (sensitivity + specificity).
