Article Figures & Data
Figures
- Fig 1
Viability and recovery of cryopreserved PBMC from 75 HIV-uninfected and 76 HIV-infected participants across the four MACS sites. The solid lines represent the median, and the dashed lines represent the mean. The box represents the 25th to 75th percentiles. The lower and upper horizontal bars represent the 10th and 90th percentiles, respectively.
- Fig 2
Viable cell recovery from each vial of cryopreserved PBMC from 75 HIV-uninfected and 76 HIV-infected participants. Numbers on the x axis represent sequential times when cryopreserved PBMC from the four MACS sites were tested. The QA testing program was started in July 2004 and was performed every 4 months. The first symbol in each testing round for each of the MACS sites is the value for the HIV-uninfected participants, and the second symbol is the value for the HIV-infected participants. The dashed lines represent 75% and 100% viable cell recoveries.
- Fig 3
Proliferative responses of cryopreserved PBMC (n = 28 per group) to phytohemagglutinin (PHA). The stimulation index was defined as the counts per minute in the presence of PHA divided by the counts per minute in the absence of PHA. The solid lines represent medians, and the dashed lines represent the means of the distributions. For HIV-uninfected samples the median and mean were the same. The boxes represent the 25th to 75th percentiles. The lower and upper horizontal bars indicate the 10th and 90th percentiles, respectively.
- Fig 4
Correlation between the PHA-induced proliferative response of cryopreserved PBMC and the percentage of CD4+ (top panel) and CD8+ (bottom panel) T lymphocytes for 27 HIV-uninfected and 27 HIV-infected participants. Least-squares regression lines are indicated.
- Fig 5
Deming regression analyses of expression of cell surface markers CD3 (top panel), CD4 (middle panel), and CD8 (bottom panel) on paired samples of fresh whole-blood and cryopreserved PBMC. A total of 54 paired blood samples (27 from HIV-uninfected and 27 from HIV-infected participants) from the 4 MACS sites were evaluated. The dashed lines represent the line of identity (y = x), and the solid lines represent the least-squares regression lines of the data.
Tables
- Table 1
T lymphocyte phenotypes in fresh whole-blood and cryopreserved PBMC
Percentage type CD3+ (%) CD3+ CD4+ (%) CD3+ CD8+ (%) Fresh Cryopreserved Fresh Cryopreserved Fresh Cryopreserved Mean 76 76 35 37 40 38 Minimum 47 40 10 10 18 18 Maximum 89 92 63 70 76 77 Median 76 78 36 38 39 36 IQRa 10.1 10.1 31.3 31.3 25.1 23.2 ↵a IQR is the interquartile range, the difference between the 25th and 75th percentiles.
