Article Figures & Data
Figures
- Fig 1
Total score points of patients showing different IgG immune responses. The antigens CagA, VacA, and GroEL score 2 points when positive. The antigens UreA, HcpC, and gGT score 1 point when positive. The total score (∑) reaches 9 points when all antigen bands show up, as illustrated in strip 5. The test is considered positive with a score of 2 or more. If only one antigen with 1 score point shows a band, the result is judged to be borderline and the test should be repeated.
- Fig 2
Results for detected immune responses of positive and negative sera verified by histology. The test is able to differentiate between type I and type II strain infections with the antigens CagA and VacA. In the top row, conjugate controls for detection of antibody (IgG) are included, serving as a positive control. To be able to discriminate the antigen bands from background staining, a cutoff control is included.
- Fig 3
Antigen frequencies in the positive cohort. In total, 210 H. pylori-positive patients were tested. The antigens CagA and GroEL showed the highest recognition rates. Immune responses against HcpC and gGT were rather frequent, but the antigens VacA and UreA showed relatively low recognition rates.
Tables
- Table 1
Characteristics of the 500 patients included in the study
Characteristic Value Histology Total Negative Positive Total no. (%) 290 (58.0) 210 (42.0) 500 (100) Age (yr) Mean value 48.5 52.3 50.1 SD 16.0 16.2 16.2 Minimum 18.0 18.0 18.0 Maximum 86.0 86.0 86.0 Gender [no. (%)] Female 154 (53.1) 109 (51.9) 263 (52.6) Male 136 (46.9) 101 (48.1) 237 (47.4) - Table 2
Performances of serological tests for the 500 cases included in the study
Parameter Performance [% (no./total)] recomLine recomWell Immunoblot Sensitivity 97.6 (205/210) 86.2 (181/210) 85.2 (179/210) Specificity 96.2 (279/290) 95.5 (277/290) 97.9 (284/290) PPV 94.5 92.7 96.4 NPV 98.4 91.2 90.8 - Table 3
Comparison of different serological tests (recomLine, Immunoblot Helicobacter, and recomWell) to histology for detection of H. pylori infectiona
Histology recomLine Immunoblot recomWell No./total − − − − 264/290 − − + − 2/290 − − − + 12/290 − + − − 8/290 − − + + 1/290 − + + − 3/290 − + − + 0/290 − + + + 0/290 + + + + 161/210 + + + − 18/210 + + − + 20/210 + + − − 6/210 + − − − 5/210 + − + − 0/210 + − − + 0/210 + − + + 0/210 ↵a −, negative; +, positive.
Supplemental material
Files in this Data Supplement:
- Supplemental file 1 -
Table S1. Primers used for amplification and cloning of H. pylori virulence factor genes. Fig. S2. Elution profile of the gel filtration run for HcpC and SDS-PAGE with the relevant fractions 9, 10, and 11. Fig. S3. Elution profile of the gel filtration run for recombinant gGT on the left side and SDS-PAGE with the relevant fractions. Table S4. Different expressed H. pylori proteins, the attached Tag for purification, and the purification steps with purity and concentration. Fig. S5. Evaluation process for CagA, measuring IgG antibodies from sera. Fig. S6. Evaluation process for VacA, measuring IgG antibodies from sera. Fig. S7. Evaluation process for GroEL, measuring IgG antibodies from patient sera. Fig. S8. Evaluation process for UreA, measuring IgG antibodies from sera. Fig. S9. Evaluation process for HcpC, measuring IgG antibodies from sera. Fig. S10. Evaluation process for gGT, measuring IgG antibodies from sera. Fig. S11. Evaluation process for UreB, Omp18, and ICD, measuring IgG antibodies from sera.
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- Supplemental file 1 -
